Fig. 2

Flowchart for the creation of the cAge predictor. First, DNAm data originating from Generation Scotland and 10 external datasets was pre-processed. Next, CpGs were pre-selected based on the Generation Scotland EWAS for epigenome-wide significant linear and quadratic CpG-age associations. Elastic net models were then trained and tested on the remaining features using a LOCO framework with 25-fold CV, with training on both age and log(age) as outcomes