Fig. 2

The metabolomic landscape of oesophageal squamous cell carcinoma. A Proportions of annotated metabolites identified in our study. B Volcano plots of the annotated metabolites. C A pathway-based analysis of metabolomic changes between tumour and NATs. The DA score captures the average, gross changes for all metabolites in a pathway. A score of 1 indicates that all measured metabolites in the pathway increase in the tumour compared to normal tissues, and a score of −1 indicates that all measured metabolites in a pathway decrease. Pathways with no fewer than three measured metabolites were used for the DA score calculation. D Pathway abundance (PA) scores between tumour and NATs. The PA score was calculated as the mean log2 fold change in the abundances of the measured metabolites in this pathway. E A metabolic map profiling the synthesis and degradation of several amino acids, based on metabolomics data. See also Fig. S2 and Table S5